The cell culture is of enormous importance for the research of basic organ functions and the development of test systems, e.g. for medicine. Also, the approach of cell therapy, in which tissue is regenerated with transplanted cells, is gaining more and more attention. In doing so, cell culture faces new challenges, such as accelerated cell replication or cryopreservation, which in turn can influence cell characteristics and function. We want to identify and reduce such influences in order to optimize the in vitro work with cells for medical use.