A software developed at the EMB allows the automated tracking of individual cells and the description of cell populations with unprecedented precision. This is made possible by continuously generated time lapse films of the cell lawn, which can be recorded noninvasively and during the normal cell culture routine. It creates a state description of the cell culture that even provides completely new information and thus represents an innovative high-content approach. This allows you to create growth curves, quantify mitoses and analyze the patterns of cell divisions. Image-based cytometry allows a quantitative and qualitative validation of cell cultures, ie a metric acquisition of biological processes.